TMEM65 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7491
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7491-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the TMEM65 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human SiHa cells with TMEM65 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM65 antibody.
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Flow cytometry testing of mouse ANA-1 cells with TMEM65 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM65 antibody.
3 / 4
Flow cytometry testing of rat RH35 cells with TMEM65 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM65 antibody.
4 / 4
Western blot testing of 1) human HeLa, 2) human PC-3, 3) human 293T, 4) human MCF7, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with TMEM65 antibody. Predicted molecular weight ~25 kDa, commonly observed at 20-25 kDa.
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Flow cytometry testing of human SiHa cells with TMEM65 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM65 antibody.
Flow cytometry testing of mouse ANA-1 cells with TMEM65 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM65 antibody.
Flow cytometry testing of rat RH35 cells with TMEM65 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM65 antibody.
Western blot testing of 1) human HeLa, 2) human PC-3, 3) human 293T, 4) human MCF7, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with TMEM65 antibody. Predicted molecular weight ~25 kDa, commonly observed at 20-25 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the TMEM65 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Predicted to be involved in cardiac ventricle development and regulation of cardiac conduction. Located in intercalated disc; mitochondrial inner membrane; and plasma membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ARDFIYSLHSTERSCLLKELH from the human protein were used as the immunogen for the TMEM65 antibody.
Limitation:
This TMEM65 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q6PI78