TDG Antibody / Thymine-DNA glycosylase

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NSJ Bioreagents
Product Code: NSJ-RQ7493
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7493-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the TDG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human PC-3 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
2 / 3
Flow cytometry testing of human HL60 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
3 / 3
Western blot testing of 1) human HeLa, 2) monkey COS-7 and 3) human placental tissue lysate with TDG antibody. Predicted molecular weight ~46 kDa.
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Flow cytometry testing of human PC-3 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
Flow cytometry testing of human HL60 cells with TDG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TDG antibody.
Western blot testing of 1) human HeLa, 2) monkey COS-7 and 3) human placental tissue lysate with TDG antibody. Predicted molecular weight ~46 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TDG antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q15-R370) was used as the immunogen for the TDG antibody.
Limitation:
This TDG antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q13569