Splicing regulator SDE2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7495
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7495-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SDE2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with SDE2 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 10
Immunofluorescent staining of FFPE human U-2 OS cells with SDE2 antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 10
Western blot testing of human 1) K562 and 2) U-251 cell lysate with SDE2 antibody. Predicted molecular weight ~50 kDa.
4 / 10
Western blot testing of human 1) K562, 2) HEL, 3) Jurkat and 4) ThP-1 cell lysate with SDE2 antibody. Predicted molecular weight ~50 kDa.
5 / 10
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human esophageal squamous carcinoma tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human spleen tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE human lung adenocarcinoma tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
IHC staining of FFPE human liver cancer tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 10
Flow cytometry testing of fixed and permeabilized human Jurkat cells with SDE2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDE2 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with SDE2 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with SDE2 antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) K562 and 2) U-251 cell lysate with SDE2 antibody. Predicted molecular weight ~50 kDa.
Western blot testing of human 1) K562, 2) HEL, 3) Jurkat and 4) ThP-1 cell lysate with SDE2 antibody. Predicted molecular weight ~50 kDa.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with SDE2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of fixed and permeabilized human Jurkat cells with SDE2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDE2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note:
Optimal dilution of the SDE2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Splicing regulator SDE2 enables damaged DNA binding activity. Involved in several processes, including cellular response to UV; mitotic G1 DNA damage checkpoint signaling; and protein ubiquitination. Located in several cellular components, including Golgi apparatus; cytosol; and nuclear speck.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids I10-K447) was used as the immunogen for the SDE2 antibody.
Limitation:
This SDE2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q6IQ49