ZNF609 Antibody / KIAA0295

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NSJ Bioreagents
Product Code: NSJ-RQ7496
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7496-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the KIAA0295 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with KIAA0295 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HEL and 2) A549 cell lysate with KIAA0295 antibody. Predicted molecular weight ~151 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with KIAA0295 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KIAA0295 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with KIAA0295 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEL and 2) A549 cell lysate with KIAA0295 antibody. Predicted molecular weight ~151 kDa.
Flow cytometry testing of human HepG2 cells with KIAA0295 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KIAA0295 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KIAA0295 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kruppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. zinc finger protein 609 (ZNF609) is a 1,411 amino acid member of the Kruppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZNF609 contains one C2H2-type zinc finger through which it is thought to be involved in DNA-binding and transcriptional regulation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K865-R1283) was used as the immunogen for the KIAA0295 antibody.
Limitation:
This KIAA0295 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
O15014