TRAM1L1 Antibody / Translocating chain-associated membrane protein 1-like 1

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NSJ Bioreagents
Product Code: NSJ-RQ7503
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7503-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TRAM1L1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with TRAM1L1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 6
IHC staining of FFPE human colorectal adenocarcinoma tissue with TRAM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human ovarian cancer tissue with TRAM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human liver cancer tissue with TRAM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) T-47D and 2) U-2 OS cell lysate with TRAM1L1 antibody. Predicted molecular weight ~42 kDa.
6 / 6
Flow cytometry testing of human HEL cells with TRAM1L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRAM1L1 antibody.
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Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with TRAM1L1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human colorectal adenocarcinoma tissue with TRAM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with TRAM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with TRAM1L1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) T-47D and 2) U-2 OS cell lysate with TRAM1L1 antibody. Predicted molecular weight ~42 kDa.
Flow cytometry testing of human HEL cells with TRAM1L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRAM1L1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the TRAM1L1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Predicted to be involved in protein insertion into ER membrane. Predicted to be integral component of endoplasmic reticulum membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids LQRWVEDSNIQASCMKKK from the human protein were used as the immunogen for the TRAM1L1 antibody.
Limitation:
This TRAM1L1 antibody is available for research use only.
Localization:
Cytoplasmic (ER)
Purity:
Antigen affinity purified
Uniprot #:
Q8N609