Timd2 Antibody / T-cell immunoglobulin mucin receptor 2

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NSJ Bioreagents
Product Code: NSJ-RQ7507
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7507-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Timd2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE mouse liver tissue with Timd2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 5
IHC staining of FFPE mouse liver tissue with Tmid2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat liver tissue with Tmid2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) mouse liver and 2) mouse kidney tissue lysate with Timd2 antibody. Predicted molecular weight ~34 kDa but may be observed at higher molecular weights due to glycosylation.
5 / 5
Flow cytometry testing of mouse HEPA1-6 cells with Timd2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Timd2 antibody.
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Immunofluorescent staining of FFPE mouse liver tissue with Timd2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE mouse liver tissue with Tmid2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with Tmid2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) mouse liver and 2) mouse kidney tissue lysate with Timd2 antibody. Predicted molecular weight ~34 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of mouse HEPA1-6 cells with Timd2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Timd2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the Timd2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Enables ferritin receptor activity. Acts upstream of or within iron ion transport. Located in cell surface. Is expressed in 4-cell stage embryo; liver; liver left lobe; liver right lobe; and metanephros. Human ortholog(s) of this gene implicated in atopic dermatitis. Orthologous to human HAVCR1 (hepatitis A virus cellular receptor 1).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KKVVERTRCEDQVYIIEDTPYPEE from the mouse protein were used as the immunogen for the Timd2 antibody.
Limitation:
This Timd2 antibody is available for research use only.
Localization:
Cell membrane
Purity:
Antigen affinity purified
Uniprot #:
Q8R183