ITK Antibody / IL2-inducible T-cell kinase

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NSJ Bioreagents
Product Code: NSJ-RQ7513
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7513-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IL2-inducible T-cell kinase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human spleen cancer tissue with IL2-inducible T-cell kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of human 1) Jurkat and 2) MOLT4 cell lysate with IL2-inducible T-cell kinase antibody. Predicted molecular weight ~72 kDa.
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Flow cytometry testing of human HEL cells with IL2-inducible T-cell kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL2-inducible T-cell kinase antibody.
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IHC staining of FFPE human spleen cancer tissue with IL2-inducible T-cell kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat and 2) MOLT4 cell lysate with IL2-inducible T-cell kinase antibody. Predicted molecular weight ~72 kDa.
Flow cytometry testing of human HEL cells with IL2-inducible T-cell kinase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL2-inducible T-cell kinase antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the IL2-inducible T-cell kinase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Tyrosine-protein kinase ITK/TSK, also known as interleukin-2-inducible T-cell kinase or simply ITK, is a protein that in humans is encoded by the ITK gene. It is a member of the TEC family of kinases. This gene is mapped to 5q33.3. This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein is thought to play a role in T-cell proliferation and differentiation. Furthermore, ITK is functionally important for the development and effector function of Th2 and Th17 cells.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R49-F465) was used as the immunogen for the IL2-inducible T-cell kinase antibody.
Limitation:
This IL2-inducible T-cell kinase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q08881