Plagl1 Antibody / Pleiomorphic adenoma-like protein 1

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NSJ Bioreagents
Product Code: NSJ-RQ7517
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7517-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Plagl1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE mouse RM1 cells with Plagl1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of mouse heart tissue lysate with Plagl1 antibody. Predicted molecular weight ~51 kDa.
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Flow cytometry testing of mouse ANA-1 cells with Plagl1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Plagl1 antibody.
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Immunofluorescent staining of FFPE mouse RM1 cells with Plagl1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of mouse heart tissue lysate with Plagl1 antibody. Predicted molecular weight ~51 kDa.
Flow cytometry testing of mouse ANA-1 cells with Plagl1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Plagl1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Plagl1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Zinc finger protein PLAGL1 (Pleiomorphic adenoma-like protein 1), also called Tumor suppressor ZAC and LOT 1 (Lost on transformation 1) is a protein that in humans is encoded by the PLAGL1 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within regulation of gene expression and skeletal muscle cell differentiation. Predicted to be located in Golgi apparatus and nuclear body. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; heart; and sensory organ. Orthologous to human PLAGL1 (PLAG1 like zinc finger 1).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant mouse protein (amino acids M1-Q527) was used as the immunogen for the Plagl1 antibody.
Limitation:
This Plagl1 antibody is available for research use only.
Localization:
Nuclear, possibly cytoplasmic (ER)
Purity:
Antigen affinity purified
Uniprot #:
B7ZP38