NOB1 Antibody / ART-4

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NSJ Bioreagents
Product Code: NSJ-RQ7520
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7520-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NOB1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE mouse brain tissue with NOB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat brain tissue with NOB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
Western blot testing of ) human HeLa, 2) human HepG2, 3) human K562, 4) human MCF7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with NOB1 antibody. Predicted molecular weight ~47 kDa.
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IHC staining of FFPE mouse brain tissue with NOB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with NOB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of ) human HeLa, 2) human HepG2, 3) human K562, 4) human MCF7, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with NOB1 antibody. Predicted molecular weight ~47 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NOB1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RNA-binding protein NOB1 is a protein that in humans is encoded by the NOB1 gene. In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-D389) was used as the immunogen for the NOB1 antibody.
Limitation:
This NOB1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9ULX3