WIPF1 Antibody / WASP-interacting protein

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NSJ Bioreagents
Product Code: NSJ-RQ7523
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7523-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the WIPF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HEL cells with WIPF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WIPF1 antibody.
2 / 3
Flow cytometry testing of human U-87 MG cells with WIPF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WIPF1 antibody.
3 / 3
Western blot testing of 1) human Daudi, 2) human MOLT4, 3) rat spleen, 4) mouse spleen and 5) mouse NIH 3T3 cell lysate with WIPF1 antibody. Commonly observed molecular weight: 50-55 kDa with a possible ~36 kDa isoform.
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Flow cytometry testing of human HEL cells with WIPF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WIPF1 antibody.
Flow cytometry testing of human U-87 MG cells with WIPF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= WIPF1 antibody.
Western blot testing of 1) human Daudi, 2) human MOLT4, 3) rat spleen, 4) mouse spleen and 5) mouse NIH 3T3 cell lysate with WIPF1 antibody. Commonly observed molecular weight: 50-55 kDa with a possible ~36 kDa isoform.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the WIPF1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
WAS/WASL-interacting protein family member 1, also called WAS/WASL-interacting protein (WIP), is a protein that in humans is encoded by the WIPF1 gene. This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids DSPCEDEWESRFYFHPISDL from the human protein were used as the immunogen for the WIPF1 antibody.
Limitation:
This WIPF1 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O43516