SAE1 Antibody / SUMO-1 activating enzyme subunit 1

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NSJ Bioreagents
Product Code: NSJ-RQ7525
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7525-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SAE1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 11
Immunofluorescent staining of FFPE human U-2 OS cells with SAE1 antibody (red) and Alpha Tubulin mAb (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 11
Immunofluorescent staining of FFPE human esophageal squamous carcinoma tissue with SAE1 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 11
Immunofluorescent staining of FFPE mouse testis tissue with SAE1 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
4 / 11
Immunofluorescent staining of FFPE rat testis tissue with SAE1 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
5 / 11
IHC staining of FFPE human intestinal cancer tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human ovarian cancer tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human lung cancer tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human duodenum tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
IHC staining of FFPE mouse testis tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 11
IHC staining of FFPE rat testis tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 11
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human 293T, 4) human MCF7, 5) rat testis, 6) mouse testis and 7) mouse thymus tissue lysate with SAE1 antibody. Predicted molecular weight ~38 kDa.
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Immunofluorescent staining of FFPE human U-2 OS cells with SAE1 antibody (red) and Alpha Tubulin mAb (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human esophageal squamous carcinoma tissue with SAE1 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE mouse testis tissue with SAE1 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat testis tissue with SAE1 antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human intestinal cancer tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human duodenum tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with SAE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human 293T, 4) human MCF7, 5) rat testis, 6) mouse testis and 7) mouse thymus tissue lysate with SAE1 antibody. Predicted molecular weight ~38 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SAE1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
SUMO-activating enzyme subunit 1 is a protein that in humans is encoded by the SAE1 gene. Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R33-M299) was used as the immunogen for the SAE1 antibody.
Limitation:
This SAE1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9UBE0