UBA5 Antibody / Ubiquitin-activating enzyme 5

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NSJ Bioreagents
Product Code: NSJ-RQ7530
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7530-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the UBA5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human colorectal adenocarcinoma tissue with UBA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human breast cancer tissue with UBA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human Caco-2, 2) human Jurkat, 3) human HeLa, 4) rat pancreas and 5) mouse pancreas tissue lysate with UBA5 antibody. Predicted molecular weight ~45 kDa, commonly observed at 40-50 kDa.
4 / 4
Flow cytometry testing of human PC-3 cells with UBA5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBA5 antibody.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with UBA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with UBA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Caco-2, 2) human Jurkat, 3) human HeLa, 4) rat pancreas and 5) mouse pancreas tissue lysate with UBA5 antibody. Predicted molecular weight ~45 kDa, commonly observed at 40-50 kDa.
Flow cytometry testing of human PC-3 cells with UBA5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBA5 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the UBA5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Ubiquitin-like modifier-activating enzyme 5 (UBA5), also called Ubiquitin-activating enzyme E1 domain-containing protein 1 (UBE1DC1), is a protein that in humans is encoded by the UBA5 gene. This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R37-D389) was used as the immunogen for the UBA5 antibody.
Limitation:
This UBA5 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9GZZ9