MRPP1 Antibody / NY-REN-49 / TRMT10C

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NSJ Bioreagents
Product Code: NSJ-RQ7545
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7545-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MRPP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human ovarian cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 12
IHC staining of FFPE human liver cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE human lung squamous cell carcinoma tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE human breast duct carcinoma tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE human spleen tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE human colon cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
Immunofluorescent staining of FFPE human ovarian cancer with MRPP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
9 / 12
Immunofluorescent staining of FFPE human rectal cancer with MRPP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
10 / 12
Immunofluorescent staining of FFPE human A549 cells with MRPP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
11 / 12
Western blot testing of human 1) HepG2, 2) Daudi and 3) HEL cell lysate with MRPP1 antibody. Predicted molecular weight ~47 kDa, commonly observed at 40-47 kDa.
12 / 12
Flow cytometry testing of human 293T cells with MRPP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MRPP1 antibody.
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IHC staining of FFPE human ovarian cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung squamous cell carcinoma tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast duct carcinoma tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer tissue with MRPP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human ovarian cancer with MRPP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human rectal cancer with MRPP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with MRPP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2, 2) Daudi and 3) HEL cell lysate with MRPP1 antibody. Predicted molecular weight ~47 kDa, commonly observed at 40-47 kDa.
Flow cytometry testing of human 293T cells with MRPP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MRPP1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MRPP1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The TRMT10C gene encodes 'tRNA methyltransferase 10 homolog C,' also called MRPP1 (Mitochondrial ribonuclease P protein 1) and 'Renal carcinoma antigen NY-REN-49,' the precursor of a subunit of the mitochondrial ribonuclease P, which is involved in 5' processing of mitochondrial tRNAs. The encoded protein may confer RNA-binding capacity to mitochondrial ribonuclease P and may be essential for transcript processing, RNA modification, translation and mitochondrial respiration.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids L36-K340) was used as the immunogen for the MRPP1 antibody.
Limitation:
This MRPP1 antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria), nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q7L0Y3