TJAP1 Antibody / PILT

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NSJ Bioreagents
Product Code: NSJ-RQ7550
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7550-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PILT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A549 cells with PILT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HepG2, 2) A431 and 3) U-251 cell lysate with PILT antibody. Predicted molecular weight ~62 kDa, commonly observed at 62-85 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with PILT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PILT antibody.
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Immunofluorescent staining of FFPE human A549 cells with PILT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2, 2) A431 and 3) U-251 cell lysate with PILT antibody. Predicted molecular weight ~62 kDa, commonly observed at 62-85 kDa.
Flow cytometry testing of human U-87 MG cells with PILT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PILT antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PILT antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Tight junction-associated protein 1, also called 'Protein incorporated later into tight junctions' (PILT), is a protein that in humans is encoded by the TJAP1 gene. This gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein localizes to the Golgi and may function in vesicle trafficking. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K160-N557) was used as the immunogen for the PILT antibody.
Limitation:
This PILT antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q5JTD0