TOX2 Antibody / GCX-1

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NSJ Bioreagents
Product Code: NSJ-RQ7554
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7554-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the TOX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A549 cells with TOX2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) rat thymus, 2) rat liver, 3) mouse thymus and 4) mouse liver tissue lysate with TOX2 antibody. Predicted molecular weight ~52 kDa, commonly observed at 52-70 kDa.
3 / 3
Flow cytometry testing of human MCF7 cells with TOX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOX2 antibody.
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Immunofluorescent staining of FFPE human A549 cells with TOX2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat thymus, 2) rat liver, 3) mouse thymus and 4) mouse liver tissue lysate with TOX2 antibody. Predicted molecular weight ~52 kDa, commonly observed at 52-70 kDa.
Flow cytometry testing of human MCF7 cells with TOX2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOX2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TOX2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
TOX high mobility group box family member 2 (TOX2), also known as Granulosa cell HMG box protein 1 (GCX-1), is a human gene. Enables transcription coactivator activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleoplasm.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q41-A425) was used as the immunogen for the TOX2 antibody.
Limitation:
This TOX2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q96NM4