TRMT6 Antibody

Product Code: NSJ-RQ7555

Code	Size	Price

NSJ-RQ7555-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Fluorescence-activated cell sorting (FACS)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the TRMT6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 13

IHC staining of FFPE human lung cancer tissue with TRMT6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

2 / 13

IHC staining of FFPE human breast cancer tissue with TRMT6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

3 / 13

IHC staining of FFPE human ovarian cancer tissue with TRMT6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

4 / 13

IHC staining of FFPE human cervical cancer tissue with TRMT6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

5 / 13

IHC staining of FFPE human testis cancer tissue with TRMT6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

6 / 13

IHC staining of FFPE human liver cancer tissue with TRMT6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

7 / 13

IHC staining of FFPE human colon cancer tissue with TRMT6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

8 / 13

Immunofluorescent staining of FFPE human breast cancer tissue with TRMT6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

9 / 13

Immunofluorescent staining of FFPE human ovarian cancer tissue with TRMT6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

10 / 13

Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue with TRMT6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

11 / 13

Immunofluorescent staining of FFPE human U-2 OS cells with TRMT6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

12 / 13

Western blot testing of human 1) HepG2, 2) placenta, 3) Daudi, 4) A431, 5) U-251, 6) SH-SY5Y, 7) MOLT4 and 8) HEL cell lysate with TRMT6 antibody. Predicted molecular weight ~56 kDa.

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Flow cytometry testing of human 293T cells with TRMT6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRMT6 antibody.

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Documents

Datasheet

Further Information

Application Details:

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Application Note:

Optimal dilution of the TRMT6 antibody should be determined by the researcher.

Buffer:

Lyophilized from 1X PBS with 2% Trehalose

Description:

This gene encodes 'mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6,' a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants.

Format:

Antigen affinity purified

Formulation:

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

E. coli-derived recombinant human protein (amino acids E6-K489) was used as the immunogen for the TRMT6 antibody.

Limitation:

This TRMT6 antibody is available for research use only.

Localization:

Nuclear

Purity:

Antigen affinity purified

Uniprot #:

Q9UJA5