TMC7 Antibody / Transmembrane channel-like protein 7

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NSJ Bioreagents
Product Code: NSJ-RQ7558
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7558-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TMC7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human intestinal cancer with TMC7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 5
IHC staining of FFPE human colorectal adenocarcinoma tissue with TMC7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human liver cancer tissue with TMC7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human HaCaT, 2) human HepG2, 3) human U-87 MG, 4) rat testis, 5) rat C6 and 6) mouse RAW264.7 cell lysate with TMC7 antibody. Predicted molecular weight ~84 kDa but may be observed at higher molecular weights due to glycosylation.
5 / 5
Flow cytometry testing of human SiHa cells with TMC7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMC7 antibody.
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Immunofluorescent staining of FFPE human intestinal cancer with TMC7 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human colorectal adenocarcinoma tissue with TMC7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with TMC7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HaCaT, 2) human HepG2, 3) human U-87 MG, 4) rat testis, 5) rat C6 and 6) mouse RAW264.7 cell lysate with TMC7 antibody. Predicted molecular weight ~84 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of human SiHa cells with TMC7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMC7 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TMC7 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Predicted to enable mechanosensitive ion channel activity. Predicted to be involved in ion transmembrane transport. Predicted to be integral component of plasma membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids A8-N723) was used as the immunogen for the TMC7 antibody.
Limitation:
This TMC7 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q7Z402