Retinoblastoma Antibody / Rb1
| Code | Size | Price |
|---|
| NSJ-V4326-20UG | 20 ug | £264.00 |
Quantity:
| NSJ-V4326-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: rRB1/8060
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Aliquot the recombinant Rb1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Description:
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human protein fragment of 283 amino acids was used as the immunogen for the recombinant Rb1 antibody. Its epitope is localized between amino acids 703-772.
Limitation:
This recombinant Rb1 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P06400