SMMHC Antibody / Smooth Muscle Myosin Heavy Chain

NSJ Bioreagents
Product Code: NSJ-V4420
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V4420-20UG20 ug£264.00
Quantity:
NSJ-V4420-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SM-M10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the SMMHC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 2
IHC staining of FFPE human uterus tissue with SMMHC antibody (clone SM-M10). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free SM-MHC antibody (

IHC staining of FFPE human uterus tissue with SMMHC antibody (clone SM-M10). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free SM-MHC antibody (

Documents

Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Description:
Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Crude human uterus extract was used as the immunogen for the SMMHC antibody.
Limitation:
This SMMHC antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P35749