PCNA Antibody

NSJ Bioreagents
Product Code: NSJ-V4822SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V4822SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PCNA/4540
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human Raji cell lysate with PCNA antibody (clone PCNA/4540). Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.~
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IHC staining of FFPE human tonsil tissue with PCNA antibody (clone PCNA/4540) at 2ug

Western blot testing of human Raji cell lysate with PCNA antibody (clone PCNA/4540). Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.~
IHC staining of FFPE human tonsil tissue with PCNA antibody (clone PCNA/4540) at 2ug

Documents

Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human PCNA protein was used as the immunogen for the PCNA antibody.
Limitation:
This PCNA antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P12004