CD133 Antibody / PROM1 / Extracellular Region

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

CD133, also known as PROML1 or Prominin, is a stem cell antigen that may be useful for the selection and expansion of hematopoietic cells and may be used as a positive marker for the characterization of trophoblast cell lines. The human CD133 gene maps to chromosome 4p15.32 and encodes an 865 amino acid protein. The CD133 gene codes for a pentaspan transmembrane glycoprotein that is expressed on primitive hematopoietic stem, progenitor, retinoblastoma, hemangioblasts and neural stem cells and developing epithelium. The 5-TM structure includes an extracellular N-terminus, two short intra- cellular loops, two large extracellular loops and an intracellular C-terminus. CD133 is a candidate gene for retinal proteins that are targeted to plasma membrane protrusions. These retinal proteins, including CD133, may influence the shedding of photoreceptive membranes from the terminal end of the outer segments of vertebrate photoreceptors, where they are phagocytosed by the retinal pigment epithelium, and represent candidates for inherited retinal degenerations.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Recombinant chimeric protein (sequences within amino acids 180-380 and 612-765) was used as the immunogen for the Prominin-1 antibody. This Prominin-1 antibody binds both glycosylated and non-glycosylated extracellular domains of Prominin-1.

This Prominin-1 antibody is available for research use only.

Cytoplasm (ER)

Protein A/G affinity

O43490