HSP90 beta Antibody / HSP90AB1

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NSJ Bioreagents
Product Code: NSJ-V5122
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V5122-20UG20 ug£264.00
Quantity:
NSJ-V5122-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: HSP90AB1/3955
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HSP90 beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells with HSP90 beta antibody (Green, clone HSP90AB1/3955) and RedDot nuclear stain (Red).
2 / 5
IHC staining of FFPE human prostate tissue with HSP90 beta antibody (clone HSP90AB1/3955). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE horse brain with HSP90 beta antibody (clone HSP90AB1/3955). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE cow brain with HSP90 beta antibody (clone HSP90AB1/3955). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 5
Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using HSP90 beta antibody (clone HSP90AB1/3955). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (clone mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
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Immunofluorescent staining of PFA-fixed human HeLa cells with HSP90 beta antibody (Green, clone HSP90AB1/3955) and RedDot nuclear stain (Red).
IHC staining of FFPE human prostate tissue with HSP90 beta antibody (clone HSP90AB1/3955). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE horse brain with HSP90 beta antibody (clone HSP90AB1/3955). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE cow brain with HSP90 beta antibody (clone HSP90AB1/3955). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using HSP90 beta antibody (clone HSP90AB1/3955). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (clone mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Documents

Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the HSP90 beta antibody.
Limitation:
This HSP90 beta antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein A/G affinity
Uniprot #:
P08238