HLA-ABC Antibody (MHC I)
Code | Size | Price |
---|
NSJ-V5178-20UG | 20 ug | £264.00 |
Quantity:
NSJ-V5178-100UG | 100 ug | £534.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: Bra-23/9
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
Storage:
Aliquot the HLA-ABC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Immunoprecipitation of lactoperoxidase radioiodinated cell surface proteins and surface sialoglycoproteins radiolabeled by sodium metaperiodate/tritiated sodium borohydride technique confirmed the structure gp44,p12 (consisting of a 44kDa glycopeptide linked with a non-glycosylated 12kDa peptide) typical for MHC class I antigen(s) as a structure recognized by Bra23/9. Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Human REH cells (a pre-B leukaemia cell line) was used as the immunogen for the HLA-ABC antibody.
Limitation:
This HLA-ABC antibody is available for research use only.
Localization:
Cell Surface
Purity:
Protein A/G affinity
Uniprot #:
P01889, P30443, P30499