Estrogen Receptor alpha Antibody

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. The receptor molecule has three domains, i.e. the central DNA-binding domain, the hormone-binding domain at the C-terminal, and the transcription-activating domain at the N-terminal. ER mediates regulatory functions of female sex steroids, mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems. Studies have shown ER Alpha is present in the nuclei of epithelial cells in normal breast and endometrial tissues, as well as a subset of breast carcinomas. Studies with immunohistochemical assay show that positive steroid hormone status has predicted favorable overall, survival, independently of hormonal treatment. Secondly, ER Alpha can be used as a tumor marker, preferentially in combination with an antibody to progesterone receptor, e.g., in the classification of adenocarcinomas.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

A recombinant partial protein sequence (within amino acids 495-595) from the human protein was used as the immunogen for the ER-A antibody.

This ER-A antibody is available for research use only.

Nucleus

Protein A/G affinity

P03372