CA3 Antibody / Carbonic Anhydrase III

NSJ Bioreagents
Product Code: NSJ-V5251SAF
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V5251SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CA3/7884
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the Carbonic Anhydrase 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human skeletal muscle tissue with Carbonic Anhydrase 3 antibody (clone CA3/7884). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2

IHC staining of FFPE human skeletal muscle tissue with Carbonic Anhydrase 3 antibody (clone CA3/7884). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA III (carbonic anhydrase III), also known as Car3 or CA3, is a 260 amino acid cytoplasmic protein that is specifically expressed in muscle. Belonging to the a-carbonic anhydrase family, CA III is activated by proton donors such as imidazole and dipeptide histidylhistidine, and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant fragment of human CA3 protein was used as the immunogen for the Carbonic Anhydrase 3 antibody.
Limitation:
This Carbonic Anhydrase 3 antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P07451