PAPP-A Antibody / Pappalysin-1

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NSJ Bioreagents
Product Code: NSJ-V5305
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V5305-20UG20 ug£264.00
Quantity:
NSJ-V5305-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PAPPA/8804R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the Pappalysin-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human placental tissue with Pappalysin-1 antibody (clone PAPPA/8804R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
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IHC staining of FFPE human placental tissue with Pappalysin-1 antibody (clone PAPPA/8804R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
Pregnancy Associated Plasma Protein (PAPP-A) is found in maternal blood that increases as pregnancy progresses, although it is not specific to pregnancy. It is principally expressed in the syncytiotrophoblast of the placenta, which forms the main source of circulating maternal PAPP-A. It cleaves insulin-like growth factor binding proteins (IGFBPs), IGFBP-4 and IGFBP-5. IGFBP-4 cleavage is enhanced significantly in the presence of bound IGF, whereas IGFBP-5 cleavage is inhibited slightly by IGF presence. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. PAPPA has also been suggested as a potential biomarker of acute myocardial infarction and Coronary Artery Disease (CAD).
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant partial protein sequence (within amino acids 351-523) from the human protein was used as the immunogen for the Pappalysin-1 antibody.
Limitation:
This Pappalysin-1 antibody is available for research use only.
Localization:
Secreted
Purity:
Protein A/G affinity
Uniprot #:
Q13219