PD-L1 Antibody / B7-H1 / CD274

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NSJ Bioreagents
Product Code: NSJ-R20345
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20345-0.1ML100 ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM320
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant PD-L1 antibody at -20°C.

Images

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Western blot testing of human tumor cell line lysate with recombinant PD-L1 antibody at 1:1000. DU145 and LNCaP are prostate cancer cell lines with very minor PD-L1 expression. Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
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IHC staining of FFPE human tonsil tissue with recombinant PD-L1 antibody at 1:250.
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IHC staining of FFPE human tonsil tissue with recombinant PD-L1 antibody at 1:250.
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IHC staining of FFPE human lung cancer tissue with recombinant PD-L1 antibody at 1:1000.
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Western blot testing of human tumor cell line lysate with recombinant PD-L1 antibody at 1:1000. DU145 and LNCaP are prostate cancer cell lines with very minor PD-L1 expression. Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated).
IHC staining of FFPE human tonsil tissue with recombinant PD-L1 antibody at 1:250.
IHC staining of FFPE human tonsil tissue with recombinant PD-L1 antibody at 1:250.
IHC staining of FFPE human lung cancer tissue with recombinant PD-L1 antibody at 1:1000.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:200-1:1000,Western blot: 1:500-1:1000,Immunocytochemistry: 1:200-1:500
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant PD-L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human PD-L1 (CD274, B7-H1).
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human PD-L1 was used as the immunogen for the recombinant PD-L1 antibody.
Limitation:
This recombinant PD-L1 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q9EP73