Prostate Specific Antigen Antibody / PSA

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NSJ Bioreagents
Product Code: NSJ-R20347
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20347-0.1ML100 ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM323
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Prostate Specific Antigen antibody at -20°C.

Images

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Western blot testing of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non-reduced(-) or reduced(+) conditions, using recombinant Prostate Specific Antigen antibody at 1:2500.
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IHC staining of FFPE human prostate cancer tissue with recombinant Prostate Specific Antigen antibody at 1:1000.
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A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of recombinant Prostate Specific Antigen antibody was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Western blot testing of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non-reduced(-) or reduced(+) conditions, using recombinant Prostate Specific Antigen antibody at 1:2500.
IHC staining of FFPE human prostate cancer tissue with recombinant Prostate Specific Antigen antibody at 1:1000.
A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of recombinant Prostate Specific Antigen antibody was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:500-1:1000,Western blot (Non-reduced): 1:1000-1:2500,ELISA: 1:100-1:5000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Prostate Specific Antigen antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to native human PSA (Prostate Specific Antigen), including both free (unbound) form and in a complex with ACT (alpha 1-antichymotrypsin). It may react to reduced PSA at a higher concentration.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Native PSA protein purified from human seminal fluid was used as the immunogen for the recombinant Prostate Specific Antigen antibody.
Limitation:
This recombinant Prostate Specific Antigen antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P07288