Recombinant Cytokeratin 5/6 Antibody

NSJ Bioreagents
Product Code: NSJ-R20364
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20364-0.1ML100 ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM341
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Cytokeratin 5/6 antibody at -20°C.

Images

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Western blot testing of human A431 cell lysate with recombinant Cytokeratin 5/6 antibody. Predicted molecular weight: ~62 kDa (KRT5) and ~60 kDa (KRT6).
2 / 4
IHC staining of FFPE human cervix tissue with recombinant Cytokeratin 5/6 antibody at 1:1000.
3 / 4
IHC staining of FFPE human lung squamous cell carcinoma tissue with recombinant Cytokeratin 5/6 antibody at 1:1000.
4 / 4
IHC staining of FFPE human prostate tissue with recombinant Cytokeratin 5/6 antibody at 1:1000.

Western blot testing of human A431 cell lysate with recombinant Cytokeratin 5/6 antibody. Predicted molecular weight: ~62 kDa (KRT5) and ~60 kDa (KRT6).
IHC staining of FFPE human cervix tissue with recombinant Cytokeratin 5/6 antibody at 1:1000.
IHC staining of FFPE human lung squamous cell carcinoma tissue with recombinant Cytokeratin 5/6 antibody at 1:1000.
IHC staining of FFPE human prostate tissue with recombinant Cytokeratin 5/6 antibody at 1:1000.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:500 -1:1000,Western blot: 1:2000-1:4000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 5/6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to both human CK-5 (Cytokeratin 5) and CK-6 (Cytokeratin 6).
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human Cytokeratin 5 was used as the immunogen for the recombinant Cytokeratin 5/6 antibody.
Limitation:
This recombinant Cytokeratin 5/6 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P13647, P02538