ALK Antibody / Anaplastic lymphoma kinase

NSJ Bioreagents
Product Code: NSJ-R20381
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20381-0.1ML100 ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM361
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant ALK antibody at -20°C.

Images

1 / 3
Western blot testing of the non-small cell lung cancer cell line H2228 cell lysate expressing EML4-ALK variant 3, using recombinant ALK antibody at 1:2000 dilution.
2 / 3
Immunohistochemical staining of FFPE NCI-H2228 cells (expressing EML4-ALK variant 3) and Daudi cells (ALK negative) with recombinant ALK antibody.
3 / 3
IHC staining of FFPE human brain tissue with recombinant ALK antibody at 1:1000.

Western blot testing of the non-small cell lung cancer cell line H2228 cell lysate expressing EML4-ALK variant 3, using recombinant ALK antibody at 1:2000 dilution.
Immunohistochemical staining of FFPE NCI-H2228 cells (expressing EML4-ALK variant 3) and Daudi cells (ALK negative) with recombinant ALK antibody.
IHC staining of FFPE human brain tissue with recombinant ALK antibody at 1:1000.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:500-1:1000,Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts human total ALK protein including ALK fusion proteins.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of human ALK (Anaplastic lymphoma kinase) was used as the immunogen for the recombinant ALK antibody.
Limitation:
This recombinant ALK antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q9UM73