c-Fos Antibody

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NSJ Bioreagents
Product Code: NSJ-R20393
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20393-0.1ML100 ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM374
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant c-Fos antibody at -20°C.

Images

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Western blot testing of lysate from HeLa cells, non-treated (-)or treated (+) with TPA, with recombinant c-Fos antibody at 1:5000 dilution. Expected molecular weight: ~40 kDa (unmodified), 53-68 kDa (phosphorylated).~
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IHC staining of FFP
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Western blot testing of lysate from HeLa cells, non-treated (-)or treated (+) with TPA, with recombinant c-Fos antibody at 1:5000 dilution. Expected molecular weight: ~40 kDa (unmodified), 53-68 kDa (phosphorylated).~
IHC staining of FFP

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:500-1:1000,Western blot: 1:1000-1:5000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant c-Fos antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human Proto-oncogene c-Fos.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to N-terminus of human Proto-oncogene c-Fos was used as the immunogen for the recombinant c-Fos antibody.
Limitation:
This recombinant c-Fos antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P01100