DDDDK Tag Antibody / FLAG Tag

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NSJ Bioreagents
Product Code: NSJ-R20415
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20415-100UG100 ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM399
Regulatory Status: RUO
Application: Western Blot (WB)
Storage:
Store the recombinant DDDDK Tag antibody at -20°C.

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Western blot testing of 293T cells (1) transfected or (2) non-transfected with a DNA construct encoding DDDDK tag fusion protein, using recombinant DDDDK Tag antibody at 0.001ug/ml.
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Western blot testing of 293T cells (1) transfected or (2) non-transfected with a DNA construct encoding DDDDK tag fusion protein, using recombinant DDDDK Tag antibody at 0.001ug/ml.

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Further Information

Application Details:
Western blot: 0.001ug/ml-0.1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant DDDDK Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to recombinant proteins containing a DDDDK fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
Mixture of a peptide with DDDDK-Tag at the N-terminal and another peptide with DDDDK-Tag at the C-terminal was used as the immunogen for the DDDDK Tag antibody.
Limitation:
This recombinant DDDDK Tag antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant