H3K27cr Antibody / Crotonylated-Histone H3 Lysine 27

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NSJ Bioreagents
Product Code: NSJ-R20417
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20417-100UG100 ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM401
Regulatory Status: RUO
Target Species: Human
Applications:
  • Dot Blot
  • Western Blot (WB)
Storage:
Store the recombinant H3K27cr antibody at -20°C.

Images

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Western blot testing of acid extracts of HeLa cells with or without sodium crotonate treatment using recombinant H3K27cr at 0.5 ug/ml.~
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A peptide dot blot showing recombinant H3K27cr antibody reacts specifically to Histone H3 crotonylate
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Western blot testing of acid extracts of HeLa cells with or without sodium crotonate treatment using recombinant H3K27cr at 0.5 ug/ml.~
A peptide dot blot showing recombinant H3K27cr antibody reacts specifically to Histone H3 crotonylate

Documents

Further Information

Application Details:
Western blot: 0.5ug/ml-2ug/ml,Dot blot: 0.5ug/ml-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K27cr antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to Histone H3 crotonylated at Lysine 27 (K27cr), and does not cross-react with acetylated or butyrylated Lysine 27. No cross reactivity with other crotonylated Lysines in histone H3.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A crotonyl-peptide corresponding to Crotonyl-Histone H3 (Lys27) was used as the immunogen for the recombinant H3K27cr antibody.
Limitation:
This recombinant H3K27cr antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P84243