Ribonuclease H2 subunit B Antibody / RNASEH2B

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NSJ Bioreagents
Product Code: NSJ-R20448
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20448-0.1ML100 ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM433
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Ribonuclease H2 subunit B antibody at -20°C.

Images

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IHC staining of FFPE human tonsil tissue with recombinant Ribonuclease H2 subunit B antibody at 1:100.
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IHC staining of FFPE human lung cancer tissue with recombinant Ribonuclease H2 subunit B antibody at 1:100.
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IHC staining of FFPE human colon cancer tissue with recombinant Ribonuclease H2 subunit B antibody at 1:100.
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Western blot testing of human Jurkat cell lysate with recombinant Ribonuclease H2 subunit B antibody at 1:1000 dilution. Predicted molecular weight ~35 kDa.
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Western blot testing of lysate from human Hela cells treated with RNAseH2B siRNA/RNAiMaxx for 72 hours. Courtesy of Dr. Antje Neeb, The Institute of Cancer Research, London, UK
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IHC staining of FFPE human tonsil tissue with recombinant Ribonuclease H2 subunit B antibody at 1:100.
IHC staining of FFPE human lung cancer tissue with recombinant Ribonuclease H2 subunit B antibody at 1:100.
IHC staining of FFPE human colon cancer tissue with recombinant Ribonuclease H2 subunit B antibody at 1:100.
Western blot testing of human Jurkat cell lysate with recombinant Ribonuclease H2 subunit B antibody at 1:1000 dilution. Predicted molecular weight ~35 kDa.
Western blot testing of lysate from human Hela cells treated with RNAseH2B siRNA/RNAiMaxx for 72 hours. Courtesy of Dr. Antje Neeb, The Institute of Cancer Research, London, UK

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:50-1:100,Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Ribonuclease H2 subunit B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human RNASEH2B/Ribonuclease H2 subunit B protein.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the C-terminus of RNASEH2B was used as the immunogen for the recombinant Ribonuclease H2 subunit B antibody.
Limitation:
This recombinant Ribonuclease H2 subunit B antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
Q5TBB1