Phospho-EIF4E Antibody (pSer209)

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NSJ Bioreagents
Product Code: NSJ-R20466
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20466-0.1ML100 ul£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM452
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store the recombinant phospho-eIF4E antibody at -20°C.

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Western blot testing of lysate of human MCF-7 cells, non-treated (-) or treated (+) with fetal bovine serum, using recombinant phospho-EIF4E antibody at a 1:1000 dilution.
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Western blot testing of lysate of human MCF-7 cells, non-treated (-) or treated (+) with fetal bovine serum, using recombinant phospho-EIF4E antibody at a 1:1000 dilution.

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Further Information

Application Details:
Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human eIF4E only when phosphorylated at Ser209. There is no cross-reactivity to eIF4E that is not phosphorylated.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
The amino acid sequence surrounding pSer209 of human EIF4E protein was used as the immunogen for the phospho-EIF4E antibody.
Limitation:
This recombinant phospho-EIF4E antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P06730