H4K12me1 Antibody / Monomethyl-Histone H4 Lysine 12

NSJ Bioreagents
Product Code: NSJ-R20472
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20472-100UG100 ug£581.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM458
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Store the recombinant H4K12me1 antibody at -20°C.

Images

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Western blot testing of recombinant Histone H4 (1) and acid extracts of human HeLa cells using recombinant H4K12me1 antibody at 0.02 ug/ml.~
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This recombinant H4K12me1 antibody specifically reacts to Histone H4 monomethylated at Lysine 12

Western blot testing of recombinant Histone H4 (1) and acid extracts of human HeLa cells using recombinant H4K12me1 antibody at 0.02 ug/ml.~
This recombinant H4K12me1 antibody specifically reacts to Histone H4 monomethylated at Lysine 12

Documents

Further Information

Application Details:
Western blot: 0.01ug/ml-0.5ug/ml,ELISA: 0.5ug/ml-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H4K12me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to Histone H4 monomethylated at Lysine 12 (K12me1). No cross reactivity with non-modified Lysine 12, or other monomethylations in Histone H4.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A monomethyl-lysine 12 peptide corresponding to Histone H4 was used as the immunogen for the recombinant H4K12me1 antibody.
Limitation:
This recombinant H4K12me1 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P62805