Phosphorylated Tau antibody (pSer396)

NSJ Bioreagents
Product Code: NSJ-R20474
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R20474-0.1ML100 ul£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: RM461
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the recombinant Phosphorylated Tau antibody at -20°C.

Images

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IHC staining of FFPE human colon tissue with recombinant Phosphorylated Tau antibody at 1:50.
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IHC staining of FFPE human brain tissue with recombinant Phosphorylated Tau antibody at 1:50.
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Western blot of mouse brain tissue lysate using recombinant Phosphorylated Tau antibody at a 1:1000 dilution. The phospho-specificity of the antibody was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Ser396.

IHC staining of FFPE human colon tissue with recombinant Phosphorylated Tau antibody at 1:50.
IHC staining of FFPE human brain tissue with recombinant Phosphorylated Tau antibody at 1:50.
Western blot of mouse brain tissue lysate using recombinant Phosphorylated Tau antibody at a 1:1000 dilution. The phospho-specificity of the antibody was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Ser396.

Documents

Further Information

Application Details:
Immunohistochemistry (FFPE): 1:50-1:100,Western blot: 1:1000-1:2000.
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Phosphorylated Tau antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to Tau only when phosphorylated at Ser396.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to human Tau (Ser396) was used as the immunogen for the recombinant Phosphorylated Tau antibody.
Limitation:
This recombinant Phosphorylated Tau antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P10636