ACTA2 Antibody

NSJ Bioreagents
Product Code: NSJ-R30094
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30094-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 1A4
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ACTA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human placental tissue with ACTA2 antibody (green), CD31 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE rat lung tissue with ACTA2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 9
IHC staining of FFPE human esophagus squama cancer tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human endometrial carcinoma tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human placental tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human lung cancer tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
IHC staining of FFPE mouse pancreas tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 9
IHC staining of FFPE rat lung tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 9
Western blot testing of 1) human placenta, 2) rat stomach and 3) mouse stomach tissue lysate with ACTA2 antibody.

Immunofluorescent staining of FFPE human placental tissue with ACTA2 antibody (green), CD31 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat lung tissue with ACTA2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human esophagus squama cancer tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial carcinoma tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse pancreas tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung tissue with ACTA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human placenta, 2) rat stomach and 3) mouse stomach tissue lysate with ACTA2 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunohistochemistry (Frozen): 2-5ug/ml,Immunofluorescence: 5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Mouse ascites fluid with 2.5% BSA and 0.025% sodium azide
Description:
Actin is a major component of the cytoskeleton and is present in most cell types.
Format:
Ascites
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 1-10 of alpha-smooth muscle actin was used as the immunogen for this ACTA2 antibody.
Limitation:
This ACTA2 antibody is available for research use only.
Purity:
Unpurified ascites