Beta-2 Microglobulin Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R32745
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32745-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Beta-2 Microglobulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE human A431 cells with Beta-2 Microglobulin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE human A431 cells with Beta-2 Microglobulin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 6
IHC testing of FFPE human intestinal cancer tissue with Beta-2 Microglobulin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 6
IHC testing of FFPE human lung cancer tissue with Beta-2 Microglobulin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 6
Western blot testing of human 1) HepG2 and 2) 22RV1 cell lysate with Beta-2 Microglobulin antibody at 0.5ug/ml. Predicted molecular weight ~14 kDa.
6 / 6
Flow cytometry testing of human U251 cells with Beta-2 Microglobulin antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta-2 Microglobulin antibody.
❮❯

Immunofluorescent staining of FFPE human A431 cells with Beta-2 Microglobulin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human A431 cells with Beta-2 Microglobulin antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal cancer tissue with Beta-2 Microglobulin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human lung cancer tissue with Beta-2 Microglobulin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of human 1) HepG2 and 2) 22RV1 cell lysate with Beta-2 Microglobulin antibody at 0.5ug/ml. Predicted molecular weight ~14 kDa.
Flow cytometry testing of human U251 cells with Beta-2 Microglobulin antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta-2 Microglobulin antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,FACS: 1-3ug/10^6 cells
Application Note:
Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q22-M119 from the human protein were used as the immunogen for the Beta-2 Microglobulin antibody.
Limitation:
This Beta-2 Microglobulin antibody is available for research use only.
Purity:
Antigen affinity
Uniprot #:
P61769