CANX Antibody / Calnexin

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ7262
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7262-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 8D10B3
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CANX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human HeLa cells with CANX antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC staining of FFPE human lung cancer tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human endometrial adenocarcinoma tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
Western blot testing of 1) human Jurkat, 2) human placenta, 3) rat brain, 4) rat liver, 5) mouse brain and 6) mouse liver tissue lysate with CANX antibody. Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
5 / 7
Flow cytometry testing of human A549 cells with CANX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CANX antibody.
6 / 7
IHC staining of FFPE mouse brain tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE rat brain tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
❮❯

Immunofluorescent staining of FFPE human HeLa cells with CANX antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial adenocarcinoma tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human placenta, 3) rat brain, 4) rat liver, 5) mouse brain and 6) mouse liver tissue lysate with CANX antibody. Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Flow cytometry testing of human A549 cells with CANX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CANX antibody.
IHC staining of FFPE mouse brain tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with CANX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the CANX antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Calnexin, also called CANX and CNX, is a 67 kDa integral protein of the endoplasmic reticulum. This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E68-R582) was used as the immunogen for the CANX antibody.
Limitation:
This CANX antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P27824