TP53 Antibody / Cellular tumor antigen p53

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NSJ Bioreagents
Product Code: NSJ-RQ7263
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7263-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TP53 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human colorectal adenocarcinoma tissue with TP53 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human esophageal squamous carcinoma tissue with TP53 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) A431, 2) T-47D and 3) 293T cell lysate with TP53 antibody. Expected molecular weight ~53 kDa.
4 / 4
Flow cytometry testing of human A431 cells with TP53 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TP53 antibody.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with TP53 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with TP53 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A431, 2) T-47D and 3) 293T cell lysate with TP53 antibody. Expected molecular weight ~53 kDa.
Flow cytometry testing of human A431 cells with TP53 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TP53 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TP53 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-D186) was used as the immunogen for the TP53 antibody.
Limitation:
This TP53 antibody is available for research use only.
Localization:
Nuclear, some cytoplasmic expression
Purity:
Antigen affinity purified
Uniprot #:
P04637