Interferon regulatory factor 7 Antibody / IRF7

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NSJ Bioreagents
Product Code: NSJ-RQ7267
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7267-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Interferon regulatory factor 7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human spleen tissue with Interferon regulatory factor 7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 293T cell lysate with Interferon regulatory factor 7 antibody. Predicted molecular weight ~54 kDa but may be observed at higher molecular weights due to phosphorylation.
3 / 3
Flow cytometry testing of human JK cells with Interferon regulatory factor 7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Interferon regulatory factor 7 antibody.
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IHC staining of FFPE human spleen tissue with Interferon regulatory factor 7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 293T cell lysate with Interferon regulatory factor 7 antibody. Predicted molecular weight ~54 kDa but may be observed at higher molecular weights due to phosphorylation.
Flow cytometry testing of human JK cells with Interferon regulatory factor 7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Interferon regulatory factor 7 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Interferon regulatory factor 7 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Interferon regulatory factor 7, also known as IRF7, is a member of the interferon regulatory factor family of transcription factors. This gene encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. It has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. The encoded protein plays an important role in the innate immune response against DNA and RNA viruses.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y27-A503) was used as the immunogen for the Interferon regulatory factor 7 antibody.
Limitation:
This Interferon regulatory factor 7 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q92985