Histone deacetylase 1 Antibody / HDAC1

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ7277
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7277-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Histone deacetylase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human tonsil tissue with Histone deacetylase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human lung cancer tissue with Histone deacetylase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human esophageal squamous carcinoma tissue with Histone deacetylase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) MOLT4, 2) Jurkat, 3) HL60 and 4) U-251 cell lysate with Histone deacetylase 1 antibody. Predicted molecular weight 55~60 kDa.
5 / 5
Flow cytometry testing of human MCF7 cells with Histone deacetylase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Histone deacetylase 1 antibody.
❮❯

IHC staining of FFPE human tonsil tissue with Histone deacetylase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Histone deacetylase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with Histone deacetylase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) MOLT4, 2) Jurkat, 3) HL60 and 4) U-251 cell lysate with Histone deacetylase 1 antibody. Predicted molecular weight 55~60 kDa.
Flow cytometry testing of human MCF7 cells with Histone deacetylase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Histone deacetylase 1 antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Histone deacetylase 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Histone deacetylase 1 is an enzyme that in humans is encoded by the HDAC1 gene. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. Furukawa et al.(1996) mapped RPD3L1 to 1p34.1 by fluorescence in situ hybridization.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids DDESYEAIFKPVMSKVMEMFQ were used as the immunogen for the Histone deacetylase 1 antibody.
Limitation:
This Histone deacetylase 1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q13547