TER ATPase Antibody / VCP / Valosin-containing protein

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NSJ Bioreagents
Product Code: NSJ-RQ7287
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7287-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TER ATPase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat brain, 2) rat lung, 3) rat stomach, 4) rat C6, 5) mouse brain, 6) mouse lung, 7) mouse stomach and 8) mouse RAW264.7 cell lysate with TER ATPase antibody. Expected molecular weight: 89-97 kDa.
2 / 8
Western blot testing of 1) human HeLa, 2) human SH-SY5Y, 3) human MCF7, 4) human A431, 5) monkey COS-7, 6) human K562, 7) human placenta and 8) human HepG2 cell lysate with TER ATPase antibody. Expected molecular weight: 89-97 kDa.
3 / 8
IHC staining of FFPE mouse colon tissue with TER ATPase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE rat colon tissue with TER ATPase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
Flow cytometry testing of human MCF7 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
6 / 8
Flow cytometry testing of human U-87 MG cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
7 / 8
Flow cytometry testing of mouse EL-4 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
8 / 8
Flow cytometry testing of rat C6 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
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Western blot testing of 1) rat brain, 2) rat lung, 3) rat stomach, 4) rat C6, 5) mouse brain, 6) mouse lung, 7) mouse stomach and 8) mouse RAW264.7 cell lysate with TER ATPase antibody. Expected molecular weight: 89-97 kDa.
Western blot testing of 1) human HeLa, 2) human SH-SY5Y, 3) human MCF7, 4) human A431, 5) monkey COS-7, 6) human K562, 7) human placenta and 8) human HepG2 cell lysate with TER ATPase antibody. Expected molecular weight: 89-97 kDa.
IHC staining of FFPE mouse colon tissue with TER ATPase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with TER ATPase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human MCF7 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
Flow cytometry testing of human U-87 MG cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
Flow cytometry testing of mouse EL-4 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
Flow cytometry testing of rat C6 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TER ATPase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Valosin-containing protein also called CDC48 is an enzyme that in humans is encoded by the VCP gene. It is a member of the AAA+ (ATPase associated with various activities) protein family. The VCP gene maps to chromosome 9p13.3. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It is involved in the formation of the transitional endoplasmic reticulum. This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. It also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D10-K512) was used as the immunogen for the TER ATPase antibody.
Limitation:
This TER ATPase antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P55072