NKX2.5 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7290
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7290-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the NKX2.5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human SiHa cells with NKX2.5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human 293T cells with NKX2.5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NKX2.5 antibody.
3 / 3
Western blot testing of human 1) U-2 OS, 2) U-251 and 3) SiHa cell lysate with NKX2.5 antibody. Predicted molecular weight ~35 kDa, routinely observed at 35~45 kDa.
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Immunofluorescent staining of FFPE human SiHa cells with NKX2.5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human 293T cells with NKX2.5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NKX2.5 antibody.
Western blot testing of human 1) U-2 OS, 2) U-251 and 3) SiHa cell lysate with NKX2.5 antibody. Predicted molecular weight ~35 kDa, routinely observed at 35~45 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NKX2.5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Homeobox protein Nkx-2.5, also known as NKX2E or CSX is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2.5 and Tbx5 directly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. The cardiac homeobox protein Nkx2.5 is essential in cardiac development, and mutations in CSX (which encodes Nkx2.5) cause various congenital heart malformations.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K15-R132) was used as the immunogen for the NKX2.5 antibody.
Limitation:
This NKX2.5 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P52952