Epoxide hydrolase Antibody / EPHX1

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NSJ Bioreagents
Product Code: NSJ-RQ7293
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7293-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Epoxide hydrolase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human 293T cells with Epoxide hydrolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Epoxide hydrolase antibody.
2 / 3
Flow cytometry testing of human RT4 cells with Epoxide hydrolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Epoxide hydrolase antibody.
3 / 3
Western blot testing of 1) human HepG2, 2) human HCCT, 3) human HCCP, 4) rat liver, 5) rat RH35, 6) mouse liver and 7) mouse HEPA1-6 cell lysate with Epoxide hydrolase antibody. Predicted molecular weight ~53 kDa.
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Flow cytometry testing of human 293T cells with Epoxide hydrolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Epoxide hydrolase antibody.
Flow cytometry testing of human RT4 cells with Epoxide hydrolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Epoxide hydrolase antibody.
Western blot testing of 1) human HepG2, 2) human HCCT, 3) human HCCP, 4) rat liver, 5) rat RH35, 6) mouse liver and 7) mouse HEPA1-6 cell lysate with Epoxide hydrolase antibody. Predicted molecular weight ~53 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Epoxide hydrolase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Epoxide hydrolase 1 is an enzyme encoded by the EPHX1 gene in humans. Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A42-Q455) was used as the immunogen for the Epoxide hydrolase antibody.
Limitation:
This Epoxide hydrolase antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P07099