PANX1 Antibody / Pannexin 1

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NSJ Bioreagents
Product Code: NSJ-RQ7295
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7295-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the PANX1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human MCF7 cells with PANX1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Flow cytometry testing of human MCF7 cells with PANX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PANX1 antibody.
3 / 4
Flow cytometry testing of human SiHa cells with PANX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PANX1 antibody.
4 / 4
Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) rat brain, 5) rat C6, 6) mouse brain and 7) mouse NIH 3T3 cell lysate with PANX1 antibody. Predicted molecular weight ~48 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with PANX1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human MCF7 cells with PANX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PANX1 antibody.
Flow cytometry testing of human SiHa cells with PANX1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PANX1 antibody.
Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) rat brain, 5) rat C6, 6) mouse brain and 7) mouse NIH 3T3 cell lysate with PANX1 antibody. Predicted molecular weight ~48 kDa.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PANX1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Pannexin 1 is a protein in humans that is encoded by the PANX1 gene. The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-D362) was used as the immunogen for the PANX1 antibody.
Limitation:
This PANX1 antibody is available for research use only.
Localization:
Cytoplasm, cell membrane, cell junction
Purity:
Antigen affinity purified
Uniprot #:
Q96RD7