PDCD4 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ7299
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7299-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the PDCD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human HepG2 cells with PDCD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PDCD4 antibody.~
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Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat and 4) T-47D cell
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Flow cytometry testing of human HepG2 cells with PDCD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PDCD4 antibody.~
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat and 4) T-47D cell

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PDCD4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Programmed cell death protein 4 (PDCD4) is a protein that in humans is encoded by the PDCD4 gene. It is mapped to 10q25.2. This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. It also encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids YDVEEVDVKDPNYDDDQENCVYE were used as the immunogen for the PDCD4 antibody.
Limitation:
This PDCD4 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
Q53EL6