JUP Antibody / Junction Plakoglobin / Gamma Catenin

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NSJ Bioreagents
Product Code: NSJ-RQ7300
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7300-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 4C12D7
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the JUP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human lung adenocarcinoma tissue with JUP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with JUP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human MCF7 cells with JUP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Western blot testing of 1) human HeLa, 2) human A431, 3) human A549, 4) human MCF7, 5) rat heart and 6) mouse heart tissue lysate with JUP antibody. Expected molecular weight: ~86 kDa.
5 / 5
Flow cytometry testing of human MCF7 cells with JUP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JUP antibody.
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IHC staining of FFPE human lung adenocarcinoma tissue with JUP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with JUP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with JUP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human A431, 3) human A549, 4) human MCF7, 5) rat heart and 6) mouse heart tissue lysate with JUP antibody. Expected molecular weight: ~86 kDa.
Flow cytometry testing of human MCF7 cells with JUP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JUP antibody.

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 2-5ug/ml, Immunofluorescence: 5ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the JUP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Junction plakoglobin (JUP), also known as gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to g-catenin, and it is mapped to 17q21.2. This gene encodes a major cytoplasmic protein that is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins. Meanwhile, JUP may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M556-A745) was used as the immunogen for the JUP antibody.
Limitation:
This JUP antibody is available for research use only.
Localization:
Cell surface, cytoplasmic, cell junctions
Purity:
Antigen affinity purified
Uniprot #:
P14923