YKL-40 Antibody / CHI3L1

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NSJ Bioreagents
Product Code: NSJ-RQ7302
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ7302-100UG100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the YKL-40 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-87 MG cells with YKL-40 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.~
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Western blot testing of 1) human U-87 MG, 2) rat liver, 3) rat RH35, 4) mouse l
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Immunofluorescent staining of FFPE human U-87 MG cells with YKL-40 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.~
Western blot testing of 1) human U-87 MG, 2) rat liver, 3) rat RH35, 4) mouse l

Documents

Further Information

Application Details:
Western blot: 0.5-1ug/ml, Immunofluorescence: 5ug/ml, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the YKL-40 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Chitinase-3-like protein 1 (CHI3L1), also known as YKL-40, is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S103-T383) was used as the immunogen for the YKL-40 antibody.
Limitation:
This YKL-40 antibody is available for research use only.
Localization:
Cytoplasm, secreted
Purity:
Antigen affinity purified
Uniprot #:
P36222